Title: The Effect of Omega-3 Therapy on Induced Hyperacidity
Authors: Hader Ibrahim Sakr, Mohamed Mansour Khalifa, Mohamed Al Sayed Saleh, Laila Ahmed Rashed, Akef Abdelhalim Khowailed
DOI: http://dx.doi.org/10.18535/jmscr/v4i2.25
Abstract
Gastric hyperacidity is a serious health problem of global concern. This work was conducted to study the effect of chronic administration of omega-3 polyunsaturated fatty acids (PUFA) on HCl secretion and stress-induced ulcer in male albino rats. Study groups included 24 rats divided into 2 main groups with 12 rats in each as follows: pyloric ligation group (A) and water immersion-induced gastric ulceration group (B). Each subgroup contained 6 rats. Among the parameters estimated in the studied groups were: free and total HCL secretion in group A, and ulcer score, ulcer index, percentage ulcer protection, histology of the stomach, eNOS enzyme activity, COX-2 enzyme activity, glutathione reductase enzyme gene expression, CCK gene expression and proton pump (H+/K+ ATPase) gene expression in group B.
RESULTS showed significant decrease in free and total acidity, ulcer score and index, COX-2 enzyme activity and Proton pump (H+/K+ ATPase) gene expression among ω-3 fed rats compared with the results of the control group. On the other hand significant elevation was observed in the percentage ulcer protection, eNOS enzyme activity, glutathione reductase gene expression and CCK gene expression among omega-3 fed rats compared to control group.
CONCLUSION, the present study revealed a protective effect of omega-3 PUFA on stress-induced gastric ulceration.
Key words: Gastric hyperacidity, HCl, H+/K+ ATPase pump, H2-receptor blockers, omega-3 PUFA.
References
1. F.A.O. (2010). Fats and fatty acids in human nutrition.Report of an expert consultation. FAO food and nutrition paper Series 91:9-10
2. Simopoulos A.P. (1999). Essential fatty acids in health and chronic disease. Am J Clin Nutr. 70(3 Suppl): 560S-9.
3. Simopoulos A.P. (2002). Omega-3 fatty acids in inflammation and autoimmune disease, J Amr Coll Nutrit. 21(6):495-505.
4. European Food Safety Authority (EFSA) (2005). Opinion of the Scientific Panel on Dietetic Products, Nutrition and Allergies on a request from the Commission related to nutrition claims concerning omega-3 fatty acids, monounsaturated fat, polyunsaturated fat and unsaturated fat. The EFSA Journal. 253:1–29.
5. Mutlu G.M., Mutlu E.A. and Factor P. (2001). GI complications in patients receiving mechanical ventilation. Chest. 119:1222–41.
6. Martindale R.G. (2005) Contemporary strategies for the prevention of stress-related mucosal bleeding. Am J Health Syst Pharm 62 (10 Suppl 2):S11-7.
7. Laine L., Takeuchi K. and Tarnawski A. (2008). Gastric mucosal defense and cytoprotection: benchto bedside. Gastroenterology 135:41–60.
8. Appleyard C.B., McCafferty D.M., Tigley A.W., Swain M.G. and Wallace J.L. (1996). Tumor necrosis factor mediation of NSAID-induced gastric damage: role of leukocyte adherence. Am J Physiol 270:G42–8.
9. Murison R. and Overmier J.B. (1993). Some psychosomatic causal factors of restraint-in-water stress ulcers. Physiol. Behav. 53:577-81.
10. Shu G., Qian G., Qing J., Wei H., Xue G. and Ji-Min C. (2012). Gastric mucosal damage in water immersion stress: Mechanism and prevention with GHRP-6. World J Gastroenterol.18(24):3145-55.
11. Hunter B., McDonald G.S. and Gibney M.J. (1992).The effects of acute and chronic administration of n-6 and n-3 polyunsaturated fatty acids on ethanol-induced gastric haemorrhage in rats. Br. J. Nutr. 67(3):501-7.
12. Shay H., Komarov S.A., Fels S.S., Meranze D., Gruenstein M. and Siplet H. (1945). A simple method for the uniform production of gastric ulceration in the rat. Gastroenterology, 4:43-61.
13. Hayase M. and Takeuchi K. (1986). Gastric acid secretion and lesion formation in rats under water immersion stress. Dig. Dis. Sci. 31: 166-71.
14. Suzuki Y., Hayashi M., Ito M., and Yamagami I. (1976). Anti ulcer effects of 4' (2 carboxyethyl) phenyl trans 4 aminomethyl cyclo-hexanecarboxylate hydrochloride (cetraxate) on various experimental gastric ulcers in rats. Japanese Journal of Pharmacology. 26(4):471–80.
15. Takagi, K., Okabe S. and R. Saziki. (1969). A new method for the production of chronic gastric ulcer in rats and the effect of several drugs on its healing. Jpn. J. Pharmacol. 19: 418-26.
16. Rajkapoor B., Anandan R. and Jayakar B. (2002). Antiulcer effect of Nigella sativa Linn. against gastric ulcer in rats. Curr. Sci. 82:177-9.
17. [1] Kulmacz R.J., and Land W.E.M. (1998). Requirements for hydroperoxide by the cyclooxygenase and peroxidase activities of prostaglandin H synthase.Prostaglandins 25:531-540.
18. [1] Faust T.W., Redfern J.S., Podolsky I., Lee E., Grundy S.M. and Feltman M. (1990). Effects of aspirin on gastric mucosal prostaglandin E, and F, content and on gastric mucosal injury in humans receiving fish oil or olive oil. Gastroenterology98:586-91.
19. [1] Riber C., Wojdemann M., Bisgaard T., Ingels H., Rehfeld J.F. and Olsen O. (1999). Fish oil reduces gastric acid secretion. Scand J Gastroenterol. 34:845–8.
20. [1]Salaj K., Mohammed A., Sunil S.D. and Satya V.P. (2008). Antiulcer activity of cod liver oil in rats. Indian J Pharmacol. 40(5):209–14.
21. [1] Al-Harbi M.M., Islam M.W., Al-Shabanah O.A. and Al-Gharably N.M. (1995). Effect of acute administration of fish oil (omega-3 marine triglyceride) on gastric ulceration and secretion induced by various ulcerogenic and necrotizing agents in rats. Food Chem Toxicol. 33:553–8.
22. Takada H., Takahashi M., Takada H., Kuwayama H. and Souma R. (2004). Omega. 3 polyunsaturated fatty acid and ulcer healing. Dokkyo J Med Sci. 31:17–223.
23. Li P., Lee K.Y., Chang T.M. and Chey W.Y. (1990). Hormonal mechanism of sodium oleate-stimulated pancreatic secretion in rats. Am J Physiol. 259: G960-5.
24. Shiratori K., JO Y.H., Lee K.Y., Chang T-M. and Chey W.Y. (1989). Effect of pancreatic juice and trypsin on oleic acid-stimulated pancreatic secretion and plasma secretin in dogs. Gastroenterology. 96:1330-6.
25. Olsen O., Ainsworth M., Schaffalitzky De Muckadell O.B. and Cantor P. (1989). Effects of oleic acid and oleyl alcohol on cholecystokinin and secretin in plasma and pancreatico-biliary secretion. Scand J Gastroenterol. 24: 529-532.
26. Konturek J.W., Stoll R., Gutwinska-Konturek M., Konturek S.J. and Domschke W. (1993). Cholecystokinin in the regulation of gastric acid and endocrine pancreatic secretion in humans. Scand J Gastroenterol. 28: 401-7.
27. Soll A.H., Amieian D.A., Park J., Elashoff J.D. and Yamada T. (1985). Cholecystokinin potently releases somatostatin from canine fundic mucosal cells in short-term culture. Am J Physiol; 248:G569-73.
28. Schubert M.L., Bitar K.N. and Makhlouf G.M. (1982). Regulation of gastrin and somatostatin secretion by cholinergic and non-cholinergic intramural neurons. Am J Physiol. 243:G442-7.
29. Gonzalez M.V., Yago M.D., Mañas M., Calpeña R., Díaz R., Martínez-Victoria E., and Mataix J. (1998). Role of the quality of dietary fat on the postprandial levels of secretin, cholecystokinin, and pancreatic polypeptide in humans. Ars Pharmaceutica. 39(3-4):165-73.
30. Phull P.S., Green C.J. and Jacyna M.R. (1995). A radical view of the stomach: the role of oxygen-derived free radicals and anti-oxidants in gastroduodenal disease. Eur J Gastroenterol Hepatol. 7(3):265-74.
31. Bhattacharya A., Ghosal S. and Bhattacharya S.K. (1995). Effect of fish oil on offensive and defensive factors in gastric ulceration in rats. Food Chem Toxicol. 33:553–8.
32. Ulak G., Ciçek R., Sermet A., Guzel C., Ulak M. and Denli O. (1995). Protective effect of fish oil against stress-induced gastric injury in rats. Arzneimittelforschung. 45:1174–5.
33. Divakar V., Sylvia S., Sarath B.K. and Devika T. (2012). Evaluation of gastro protective activity of Fish Oil (Omega- 3 Fatty Acids) against experimentally induced acute gastric ulceration in Rats. Int. J Med Health Sci. 1:2.
34. Ozgocmen S., Atalay Catal S., Ardicoglu O. and Kamanli A. (2000). Effect of omega-3 fatty acids in the management of fibromyalgia syndrome, Int. J. Clin. Pharm. Ther. 38:362–3.
35. James M.J., Gibson R.A. and Cleland L.G. (2000). Dietary polyunsaturated fatty acids and inflammatory mediator production. The American Journal of Clinical Nutrition. 71(1):343S–8.
36. Su K.P., Shen W.W and Huang S.Y. (2000) Are omega3 fatty acids beneficial in depression but not mania? Arch Gen Psychiatry. 57:716-7.
37. Das U.N. (2006). Essential fatty acids: biochemistry, physiology and pathology. Biotechnol J. 1:420-39.
38. Feige J.N., Gelman L., Michalik L., Desvergne B. and Wahli W. (2006). From molecular action to physiological outputs: peroxisome proliferator-activated receptors are nuclear receptors at the crossroads of key cellular functions. Progress in Lipid Research. 45(2):120–59.
39. Shimada T., Koitabashi A., Kuniyoshi T., Hashimoto T., Yoshiura K., Yoneda M., Hiraishi H. and Terano A. (2003). Up-regulation of TFF expression by PPAR-gamma ligands in gastric epithelial cells. Alimentary pharmacology &therapeutics. 18 Suppl 1:119-25.
40. Konturek P.C., Brzozowski T., Burnat G., Szlachcic A., Koziel J., Kwiecien S., Konturek S.J. and Harsch I.A. (2010). Gastric ulcer healing and stress-lesion preventive properties of pioglitazone are attenuated in diabetic rats. Journal of physiology and Pharmacology. 61(4):429-36.
41. Kawano S. and Tsuji S. (2000). Role of mucosal blood flow: a conceptional review in gastric mucosal injury and protection. J Gastroenterol Hepatol. 15(Suppl):D1-D6.
42. Tarek A. and Samar S.E. (2006). Effect of peroxisome proliferator- activated receptor-gamma (PPAR-γ) ligands on gastric mucosal injury induced by ischemia/reperfusion in rats. Bull. Alex. Fac. Med. 42(1).
43. [1] Chan J.A., Nagaswa M., Takeguchi C. and Sih C.J. (1975) on agents favoring prostaglandin E formation during biosynthesis. Biochemistry. 14:2987-91.
44. Jimmy Y.C., Chow L.M., Min Z. and Chi H.C.H. (1997). The potentiating actions of cigarette smoking on ethanol-induced gastric mucosal damage in rats. Gastroenterology. 113:1188-97.
45. Wallach D.P. and Daniele E.G. (1971). Properties of novel preparation of prostaglandin synthetase from seminal vesicles. Biochem. Biophys. Acta. 231:445-57.
46. Alarcon C., Lopez A. and Motilva V. (1993). Gastro-protection and prostaglandin E2 generation in rats by flavonoids of Dittrichia viscosa. Planta Medica. 59: 497-501.
47. Jiang C., Ting A.T. and Seed B. (1998). PPAR-gamma agonists inhibit production of monocyte inflammatory cytokines. Nature. 391:82-6.
48. Konturek P.C., Brzozowski T., Burnat G., Szlachcic A., Koziel J., Kwiecien S., Konturek S.J. and Harsch I.A. (2010). Gastric ulcer healing and stress-lesion preventive properties of pioglitazone are attenuated in diabetic rats. Journal of physiology and Pharmacology. 61(4):429-36.