Title: Quantification of microRNA-181b in Egyptian Acute Myeloid Leukemia Patients and its Relation to Prognosis
Authors: Iman M Omar, Dina A Fouad, Deena M M Habashy, Doaa G Eissa, Mariam F Abdel-Maksoud
DOI: http://dx.doi.org/10.18535/jmscr/v4i3.46
Abstract
Unique micro RNA (miRNA) expression signatures are aberrantly expressed in acute myeloid leukemia (AML). Certain miRNA signatures have been associated with prognosis of AML, one of these is miR-181b. We aimed to quantify miR-181b in AML Egyptian adult patients and evaluate its prognostic significance in those patients. Eighty newly diagnosed AML patients with 40 age and sex matching healthy volunteers as a control group were studied for the expression of miR-181b by stem-loop quantitative reverse-transcription polymerase chain reaction (qRT- PCR).miR-181b expression level was higher in patients’ group than controls (p=0.013). Patients with miR-181b ³ 6.0 were associated with favorable molecular abnormalities and most of them achieved complete remission (CR), whereas most of patients with miR-181b< 6.0 were associated with unfavorable ones and failed to achieve CR. Patients with miR- 181b values ≥ 6 showed significantly higher mean survival than patients with values <6 (35±0.7, 14.6±2 month respectively, p<0.001).Low value of miRNA181b was identified as an independent predictor of bad prognosis. High expression level of miR-181b is detected in Egyptian AML adult patients identifying a distinct group with favorable prognosis
Keywords: miR-181b, acute myeloid leukemia, prognosis
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