Title: Effects of CYP3A5 and ABCB1 Genetics Variant on Tacrolimus Pharmacokinetics in Algerian adult Kidney Transplant Patients
Authors: Fatma Boudia, Wefa Boughrara, Aberkane Meriem, Fatima Zohra Moghtit, Abdeslam Hamdani, Habiba Fetati, Fatima Zohra Mekaouche, Houari Toumi
DOI: http://dx.doi.org/10.18535/jmscr/v4i4.34
Abstract
Objective: The aim of this study is to determine the impact of CYP3A5 c. 6986A>G and ABCB1 c. 3435C>T polymorphisms on Tacrolimus (Tac) pharmacokinetics in Algerian kidney recipients transplant. Pharmacogenetics methods may be used prospectively to aid dose selection and individualize immunosuppressive therapy.
Methods: Sixty three kidney transplant patients from West Algerian population were enrolled in the study. The Tac pharmacokinetic parameters were calculated from patients blood. The Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique and data were analyzed by χ2.
Results: Our findings suggest that there is a significant distribution of TAC Concentration/Dose Ratio in Days 30 to 90 for these polymorphisms. However, at the sixth month after transplantation the Tac concentration/Dose Ratio present a significant distribution for only the CYP3A5 c. 6986A>G polymorphism.
Conclusion: We have shown, for the first time in Algerian population that theses polymorphisms are not an important genetic factor on Tac pharmacokinetics.
Keys Words: Kidney transplant, CYP3A5, ABCB1, Tacrolimus, cyclosporine, Algeria, polymorphism.
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