Title: Correlation of Fetal Hemoglobin (HbF) with Clinical Findings and red cell indices in Thalassemia: ‘Harbinger of HbF Inducer Therapies’
Authors: Sharmila Gupta, Ashok Kumar Kapoor, Vidya Bhushan, Ashutosh Kumar, Upama
DOI: https://dx.doi.org/10.18535/jmscr/v4i12.36
Aim of this study was to correlate the fetal hemoglobin (HbF) values with red cell indices in patients with thalassemia. Present study was done on 9 patients with thalassemia. All the 9 patients belonged to Tharu Chaudhary community and hailed from mid-western region of Nepal. Thirteen subjects with abnormal hemoglobin structure acted as disease-controls. Eight other subjects with normal hemoglobin structure were also taken as disease-controls. All the subjects (n = 30) included in this study had microcytic hypochromic anemia. Venous blood was collected from all the patients. Blood cells were washed and lysed. Later, Hb electrophoresis was done. Hb electrophoresis revealed presence of HbF in blood of 8 of 9 thalassemic patients. HbF values ranged from 2% to 96% in patients. HbF was inversely correlated with MCHC and MCV. HbA2 was also correlated with Hct and RBC. Findings of this study suggested that rise in HbF in thalassemic patients may improve hypochromia and thus may favor survival of red cells. 1. Lafferty JD, Crother MA, Ali MA, Levine ML. The evaluation of various mathema-tical RBC indices and their efficacy in discriminating between thalassemic and non-thalassemic microcytosis. Amer J clinpathol 1996, 106:201-5. 2. Lewis SM, Bain BJ and Bates I. Dacie and Lewis Practical Haematology, 9thed, London : Churchill Livingstone; 2001, p 275-276. 3. Fischback Talaska Frances. Dunning III Marshall Barnett: A manual of laboratory and diagnostic tests 8th ed. New Delhi :Wolters Kluwer/Lippincott Williams and Wilkins; 2009, p108-110. 4. Musallam KM, Sankaran VG, Cappellini MD, Duca L, Nathan DG, Taher AT. Fetal hemoglobin levels and morbidity in untransfused patients with β thalassemia intermedia. Blood 2012; 119(2) : 364-367. 5. Telen MJ, Kaufman RE. Wintrobe’s Clinical Hematology 10thed. Baltimore. Maryland: Williams and Wilkins; 1999, p1342-1343. 6. Wheatherall DJ, editor. The molecular basis of blood disease. 2nd ed. Philadelp-hia: WB Saunders Company; 1994, p157. 7. AsadovChD, Mamedova TA, Kulieva ED, Shakhguseinova SB. Diagnosis of β-thalassemia. Klin Lab Dign; 2004, 5:45-7. 8. Ahmed S, Saleem M, Modell B, Pertrou M. Screening extended families for genetic hemoglobin disorders in Pakistan. N Eng J Med 2003; 347: 1162-68. 9. McKerrow JH, Davies SJ. Parasitic diseases. In :Parslow TG et al editors Medical Immunology, 4th ed. New York : Elsevier; 1997, p673-75. 10. Hamilton SR, Farber JL, Rubin E : The gastrointestinal tract. In ‘Pathology’ 3rd edition, Rubin E, Farber JL (editors), Lippincott-Raven (publishers), Philadelphia, p705, 1998. 11. Bonner H, Bogg A, CossmanJ : The blood and lymphoid organs. In ‘Pathology’ 3rd edition, Rubin E, Farber JL (editors), Lippincott-Raven (publishers), Philadelphia, p1078. Dr Ashok Kumar Kapoor D87 Mahanagar Extension, Vigyan Puri, Lucknow, Uttar Pradesh, India-226006 Email: This email address is being protected from spambots. You need JavaScript enabled to view it.Abstract
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