Title: Prevalent non-tuberculous Mycobacteria (NTM) species among presumptive MTBDR patients from peripheral health facilities referred for testing at KEMRI TB Laboratory-Kisumu, Kenya
Authors: Okumu Albert, Asito Stephen, Omondi Isaiah, Ogollah Christine, Shiluli Clement, Murithi Wilfred, Odero Ronald, Malika Timothy, Wandiga Steve
DOI: https://dx.doi.org/10.18535/jmscr/v9i4.27
Abstract
Objectives: The aim of this study was to establish prevalent Nontuberculosis Mycobacteria among presumptive multi-drug resistant TB (MDRTB) patients in western Kenya. Sputum samples obtained from laboratory confirmed MDRTB patients were processed using standard culture methods, fluorescence microscopy (FM), MGIT culture, Xpert MTB/RIF. Mycobacteria tuberculosis complex (MTBc) and NTM were identified using line probe assays techniques.
Results: Between January 2012 to December 2015, a total of 287 samples from presumptive MDRTB individuals were flagged positive with liquid culture (MGIT), subsequently had line probe assay (LPA) performed for potential nontuberculous mycobacteria species. Of these, 155 (54%) had NTMs and 14 (4.87%) MTBc by LPA technique, respectively. Males constituted 140(49%) of which 64 (22.3%) were HIV infected, while females were a total of 160(55.7%), with 96 (33.4%) HIV infected. 41(14.3%) individuals with unknown HIV status. M. intracellulare 62(21.6%), and M. scrofulaceum 13(4.5%) were most prevalent NTMs species identified.
Keywords: Nontuberculous mycobacteria, multidrug resistance tuberculosis, pulmonary, diagnosis, line probe assay, microscopy.
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