Title: The Study of glucose uptake activity in Alli chooranam (Nymphea nouchali burm.f) in L6 cell lines
Authors: Janani Syamaroopa Jnanathapaswini, Manoharan.A
DOI: https://dx.doi.org/10.18535/jmscr/v9i3.25
Abstract
The Alli (Nymphea nouchali burm.f) is a well-known medicinal plant used in Siddha system of medicine for the treatment of Diabetes mellitus. The effectiveness of glucose lowering herbs is by stimulating glucose uptake by adipose and muscle tissues, hindering the glucose absorption from intestine and prohibiting glucose production from hepatocytes. This study was carried out to determine the glucose uptake activity using in vitro cell culturing methods on L6 cell lines. Treatment of the cells with plant extract significantly increased glucose uptake, the highest concentration (100 µg/ml) giving 46.2%. (P<0.05). The study concluded that aqueous extract increases glucose uptake confirms the anti-diabetic activity of Alli chooranam (N.nouchali).
Keywords: Alli, Diabetes, Glucose uptake, L6Cell line study, Nymphea nouchali, Siddha system
References
- Idowu Jonas Sagbo, Marynavan de ventor, Trevor koekomoer et al In vitro anti-diabetic activity and mechanism of action of Brachylaena elliptica (thumb.) DC, Evidence based complementary and alternative medicine, Jul 4
- Mabel parimala, Francis Gricilda Sobha 2014,Evaluation of antidiabetic potential of Nymphea nouchali Burm fseeds in STZ induced diabetic rats, International journal of pharmacy and pharmaceutical sciences, vol6(4)
- Murugesa Muthaliyar KS, 1936, Gunapadam (MutharPagam-Mooligai Vaguppu), Directorate of Indian Medicine and Homeopathy,Chennai ,2nd edition,43
- Panda H, Handbook on MedicinalHerbs with uses, Asian pacific business press
- Pulliah, Chandrasekhar Naidu, 2012, Ant diabetic plants in India, 978-81-87498-67-4.
- N. Guptha, Anilpareek, Manish Suthar, Garvendra SRathor 2009,Study of glucose uptake activity of Helictres isora Linn.fruits in L6 cell lines, Int J Diabetes Dev Ctries Oct-Dec;29(4):170-173
- Rajeev Chawla, Manual of Diabetes care, Jaypee Brothers MedicalPublishers,2014,1
- Soumya G,Dr Solomon Sunder Raj 2012,Antidiabetic activity of ethanolic extract of Nymphea lotus roots on streptozotocin induced Diabetic rats, Journal of Pharmacy research,5(9),4695-4696.
- Tafadzwa Taderera, Exnevia gomo et al, Annona stenophylla aqueous extract stimulate glucose uptake in established C2Cl2 muscle celllines, African Health Sciences,2019 Jun;19(2):2219-2229
- YamamotoN,Ueda-Wakagi M et al, Measurement of glucoseuptake inculturedcells, Curr ptotoc pharmacol, 2015,Dec 8;71:12,14,1doi 10.