Objective: Comparative study of body fluid cytology using cytospin and centrifuge.

Material and Method: Our study was carried out on 100 patients admitted in wards of Saraswathi Institute of Medical Sciences Hapur (U.P.). Samples of ascitic, pleural and cerebrospinal fluids aspirated from admitted patients .The fluids was equally divided and simultaneously centrifuged in ordinary centrifuge and Cytospin. The smears for both methods were fixed for Giemsa stain. Other stains like Pap and H&E were done only for those cases which will be positive for malignancies. Both methods were compared on the basis of Background, cell morphology, cell yield and distribution of cells.

Result: Cytospin showed better cell yield, cell morphology, cell distribution as compared to Centrifuge in pleural, peritoneal fluids and CSF but background was not comparable.

Conclusion: Cytospin was found superior to conventional centrifuge smears in terms of cell yield, preservation of cell morphology and uniformity of cell distribution. Cytospin helped in better diagnosing malignant effusions than conventional smears. Hence, cytospin can be preferred to conventional smears for cytologic examination of body cavity fluids.

Keywords: Cytospin, Centrifuge.

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Dr Prapti Gupta

Junior Resident Department of Pathology Saraswathi Institute of Medical Sciences Hapur (U.P.)