Title: Evaluation of Rapid Slide Culture Technique for the Diagnosis of Pulmonary Tuberculosis
Authors: Dr Shafikul Islam Khan, Dr Md. Akram Hossain, Dr Ummey Shahnaz Parvin, Dr Nazia Haque, Dr Nasir Uddin Mahmud, D S M Zakir Hossain
DOI: https://dx.doi.org/10.18535/jmscr/v7i11.22
Abstract
This study was conducted to evaluate the rapid slide culture technique for the diagnosis of pulmonary tuberculosis. A cross sectional study was carried out in the Department of Microbiology, Mymensingh Medical College and the Reference Laboratory of Damien Foundation at Anantapur in Netrakona, over a period of one year from July 2007 to June 2008. A total of 214 sputum sample were collected purposively from patients attending at the DOT’S corner of Mymensingh Medical College Hospital and DOT’S corner of Netrakona during the study period. Out of 214 samples, 50 (23.36%) & 42 (19.62%) showed growth on Ogawa culture and slide culture positive respectively. On the other hand Ziehl- Neelsen staining (Z-N) was positive in 46 (21.49%) of the samples. Slide culture technique showed 8 false negative and no false positive results but in Z-N staining method showed 9 false negative and 5 false positive results when compared with Ogawa culture. The sensitivity and specificity of slide culture were 84% and 100% respectively which was higher than that of microscopy by Z-N staining (82%) and 96.95% respectively.
Keywords: Pulmonary tuberculosis; Slide culture; Ogawa culture; Ziehl-Neelsen staining.
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