Background: Platelet-rich plasma (PRP) are platelets concentrates made of autogenous blood with a high number of platelets in a small volume of plasma. The main component that appears to be associated with the therapeutic effect in PRP is the presence of growth factors (GF). Variable techniques for PRP preparation produce variable amounts of growth factors.

Subjects and Methods: The study included a total number of 30 healthy males volunteer. We collected blood samples on lithium heparin, EDTA (ethylene diamine tetra acetic acid) and trisodium citrate as anticoagulants and counted platelets in whole blood and PRP which were prepared by single spin and double spin. We measured platelets derived growth factor-BB (PDGF-BB) and transforming growth factor beta-1(TGFB-1) in PRP and platelets poor plasma (PPP).

Results: We obtained high significant difference between PRP and PPP were prepared from samples on the different anticoagulants (p<0.01) for both TGFB-1 and PDGF-BB. No significant correlation between platelets counts and both TGFB-1 and PDGF-BB in PRP from the different anticoagulants samples (p>0.05). High significant difference was found between growth factors in the PRP were prepared by single spin and double spin (p<0.01) where TGFB-1 and PDGF-BB were higher in single spin PRP than that prepared by double spin.

Keywords: Anticoagulants, Platelets rich plasma, Growth factors.

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Hoda Elhady Ahmed Abdelmoneim

Egypt, Zkazik City, 4 Alhasn Bn Alhaithm Street, Alqaumia

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