Title: Role of Serum Estradiol Levels on the Day of Human Chorionic Gonadotropin Injection Administration in the Prediction of Results of Assisted Reproductive Techniques
Authors: Fessy Louis .T, Sagar S Mane, Ramesh P, Gopinathan KK
DOI: https://dx.doi.org/10.18535/jmscr/v6i9.39
Abstract
We need to find out factors which can predict the outcome of In vitro fertilization/ Intracytoplasmic sperm injection cycles so that couple can take correct decision before opting for assisted reproductive techniques. Our objective was to evaluate the role of serum estradiol level on the day of human chorionic gonadotropin administration as a potential predictive marker of outcome of IVF/ICSI cycles. This was a prospective study done in a tertiary care centre. Serum estradiol (E2) levels were obtained on the day of hCG administration. After 14 days of embryo transfer, serum beta hCG level was estimated and a value of 50 IU/L was taken as cut-off of positive cycle. The biochemical pregnancy rate was 31%. The mean value of serum estradiol was 1970.16 ± 1140.64 pg/ ml in the non pregnant group whereas that in the pregnant group was 2129.31 ± 787.91pg/ ml. (p= 0.34). The study population was divided into 5 groups based on the serum estradiol level (pg/ml) on the day of hCG administration. Number of follicles >14mm was significantly higher in groups with E2 values 3000-4000 and >4000 pg/ml (p<0.001). There was no significant difference in the endometrial thickness in the groups (p=0.131). The pregnancy rate was not found to be significantly different among the groups (p=0.34). The number of MII oocytes and the number of transferable embryos were significantly less in the groups with E2 level <1000 and 1000-2000 pg/ ml.
Therefore, serum estradiol level on the day of hCG administration was positively correlated with number of follicles with size more than 14mm obtained, metaphase II oocytes obtained, number of transferable embryos obtained. There was no association of serum estradiol level on the day of hCG administration with endometrial thickness on day of hCG and IVF outcome.
Keywords: Estradiol, Day of trigger, ART outcome, Pregnancy rate.
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