Title: A study on the expressions of Ki-67 and Cyclin-D1 in oral pre malignant lesions
Authors: Dr Ashwani Kumar Tripathi, Dr Vivek Gupta, Dr Rashmi Chaturvedi, Dr Puneet Maheshwari, Dr Ajay Kumar
DOI: https://dx.doi.org/10.18535/jmscr/v6i8.06
Abstract
Objective: To evaluate the expressions of Ki-67 and Cyclin-D1 in oral pre malignant lesions.
Methods: This was a prospective observational study. Clinical history was taken from all the cases with more stress on probable risk factors of lesions. Patients were questioned about habits of smoking, alcohol consumption and tobacco chewing. Patients were also questioned about their socioeconomic status, dietary habits and were examined for oral hygiene. Stained sections from all the formalin-fixed, paraffin embedded tissue specimens (n=44), serial sections (4-5 Im thick), were prepared and processed for routine histopathological and subsequent immunohistochemical studies.
Results: More than one third of patients were between 41-50 years (47.7%). More than one half of patients were males (65.9%).Severe dysplasias was among 34.1% patients and moderate dysplasias was in 20.5% patients. Mild dysplasias was found in 18.2% and lichen planus was in 15.9% patients. Hyperplasias was found to be in 11.4% patients. Over expression of cyclin D1 (≥10%) was found among 79.5% patients. Cyclin D1 level was <10% in 13.6% of the patients. Overexpression of Ki67 (≥10%) was found among 70.5% patients. Ki67 level was <10% in 20.5% of the patients.
Conclusion: expression of the Cyclin-D1 and Ki67 with increasing severity of dysplasia may be a prognostic indicator of any preceding malignant transformation.
Keywords: Malignant lesions,Cyclin-D1, Ki67.
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