Infections due to gram negative bacilli (GNB) are more common and increasing antibiotic resistance amongst these infections is a worldwide problem. Enterobacteriaceae are frequently isolated from various clinical samples. Overuse or irrational use of antibiotics to treat these infections lead to develop antibiotic resistance, such multidrug resistant (MDR)-Enterobacteriaceae infections are difficult to treat. The present study was conducted to know the antibiogram of Enterobacteriaceae isolated from various clinical samples. This retrospective study was conducted in Dr D Y Patil Hospital & Research Centre, Kadamwadi, Kolhapur, Maharashtra, India over a period of one year (December 2016- December 2017). Identification and antibiotic sensitivity of Enterobacteriaceae were performed by standard microbiological procedures and VITEK II automated system. A total 235 Enterobacteriaceae GNB was isolated from various clinical samples. The most frequently isolated organism was Escherichia coli 45.5% followed by Klebsiella spp 31%, Citrobacterspp 11.9%, Proteusspp 9.3%, Serratiamarcescens 1.2% & Enterobacteraerogens 0.8% in the present study. Most of the organisms were isolated from urine sample 44% followed by pus 40%. Carbapenems, Tigecycline and Amikacin were effective against MDR-Enterobacteriaceae. Most effective antibiotics were Imipenem showed 97%, Meropenem 91%, Amikacine 91%, Gentamicin 83.4%, Piperacillin-tazobactum 79.5%, Tigecycline 97% and Ciprofloxacine 41,2% sensitivity. Most resistant antibiotics were Ampicillin showed 31.4%, Ceftazidime 25.5% and Ceftrixone 24.6% sensitivity in the present study.

Keywords: Antibiogram, Enterobacteriaceae, Escherichia coli, Imipeneme.

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Dr Vishwashanti S Vatkar

Associate Professor, Microbiology Department,

D Y Patil Medical College, Kolhapur