Now-a-days infertility is an emerging problem involving the women with reproductive age group. Polycystic ovarian syndrome (PCOS) is one of the leading cause that affects etiology of infertility to a great extent. Hyperandrogenism, Amenorrhea and Polycystic ovaries characterize the disease. Long term complication include DM Type II, Obesity, Heart disease and Endometrial cancer. Our study was aimed to compare the level of Insulin Resistance (IR) and serum hsCRP (a marker of low grade inflammation) among PCOS patients and controls and specifically to determine whether there was any correlation between Insulin resistance and hs-CRP level among PCOS patients. In search of suitable markers for diagnosis as well as management of such patients, this case control study was conducted among 44 PCOS cases and 44 non-PCOS age matched controls. Serum Fasting Blood Sugar (FBS) by GOD-POD method in auto analyzer, hs-CRP and Fasting Insulin by enzyme linked immunosorbent assay were measured. Insulin resistance was calculated by HOMA-IR. Unpaired‘t’ test was used to compare different means between two groups .Statistical correlation was tested between hsCRP and Insulin resistance to find any association. Statistically significant differences of means were observed in the values of Fasting Insulin, hs-CRP and IR between cases and controls as the ‘p’ values were <0.05. No such statistically significant difference was observed between means of FBS and correlation between Insulin Resistance and hs-CRP among PCOS cases. So the study concluded that PCOS patients have high level of mean Fasting Insulin, hs-CRP and Insulin Resistance than controls.

Keywords: PCOS, FBS, Fasting insulin, hs-CRP, HOMA-IR, insulin resistance.

1. Azziz R, Sanchez LA, Knochenhauer ES, Moran C, Lazenby J, Stephens KC et al. Androgen excess in women: experience with over 1000 consecutive patients. J Clin Endocrinol Metab 2004;89:453.
2. Kollmann M, Martins WP, Raine-Fenning N. Terms and thresholds for the ultrasound evaluation of the ovaries in women with hyperandrogenic anovulation. Hum Reprod Update. 2014 May-Jun;20(3):463-4.
3. Stein IF, Leventhal ML. Amenorrhea associated with bilateral polycystic ovaries.Am J Obstet Gynecol 1935;29:181
4. Glintborg D, Andersen An update on the pathogenesis, inflammation and metabolism in hirsutism and polycystic ovary syndrome. Gynecol Endocrinol 2010;26:281-96.
5. Diamanti-Kandarakis E, Kandarakis H, Legro RS. The role of genes and environment in the etiology of PCOS. Endocrine. 2006 Aug;30(1):19-26.
6. Rotterdam ESHRE/ASRM-Sponsored PCOS Consensus Workshop Group. Revised 2003 consensus on diagnostic criteria and long-term health risks related to polycystic ovary syndrome Society. Fertil Steril. 2004;81:19–25.[PubMed]
7. I Tarkun et al. Endothelial dysfunction in young women with polycystic ovary syndrome: relationship with insulin resistance and low grade chronic inflammation. The Journal of Clinical Endocrinology and Metabolism. 2004; 89(11): 5592-5596.
8. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC (1985). "Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man.". Diabetologia. 28(7): 412–9. 
9. medscape.com/article/2094831-overviewSep 15, 2015 - The reference range for C-reactive protein and high-sensitivity C-reactive proteinare as follows CRP: 0-10mg/dL hs-CRP: < 3 mg/L
10. Steven M.HaffnerM D. The Metabolic Syndrome: Inflammation, Diabetes Mellitus, and Cardiovascular Disease. The American Journal of Cardiology Volume 97, Issue 2, Supplement 1,16January 2006, Pages 3-11
11. com/calculators/other/homa/ Category, HOMAScore. Normal insulin resistance, <3. Moderate insulin resistance, Between 3 and 5. Severe insulin resistance, >5 ...
12. Ganie MA et al. Treatment of polycystic ovary syndrome: recent trial results. Clin. Invest. (Lond.) 2015; 5(3): 337-350.
13. Nitin Agrawal et al. Polycystic ovary syndrome and insulin resistance: a north Indian study. International J. of Research in Medical Sciences. 2017; 3(6): 1321-1324.
14. Dunaif A. Insulin resistance and the polycystic ovary syndrome: mechanism and implications for pathogenesis. Endocr Rev 1997;18(6):774-800.
15. Georgios Daskalopoulos et al. Excess metabolic and cardiovascular risk is not manifested in all phenotypes of polycystic ovary syndrome: implications for diagnosis and treatment. Current vascular Pharmacology. 2015; 13(6): 788-800.
16. Makedos A et al.Increased serum C-reactive protein levels in normal weight women with polycystic ovary syndrome. Hippokratia 2011 Oct-Dec; 15(4):323-326.
17. Unni C.Sumithra et al. evaluation of oxidative stress and hs-CRP in polycystic ovarian syndrome in a tertiary care hospital. Indian J Clin Biochem. 2015 Apr; 30(2): 161-166.
18. Sapna Janjir et al. Evaluation of hsCRP, insulin and insulin resistance in polycystic ovary syndrome. IJCBR. Apr-Jun 2017; 4(2): 195-197.
19. S Sahin et al. Intrinsic factors rather than vitamin D deficiency are related to insulin resistance in lean women with polycystic ovary syndrome. Eur Rev Med Pharmacol Sci. 2014; 18(19): 2851-6.
20. Mark O Goodarzi et al. Relative impact of insulin resistance and obesity on cardiovascular risk factors in polycystic ovary syndrome. Article in Metabolism Clinical and Experimental. July 2003: 52(6):713-9.

Dr. Sujit Kumar Dey

Associate Professor, Dept. of Biochemistry, 4th Floor, Academic Building,

R. G. Kar Medical College, 1, Kshudiram Bose Sarani, Kolkata-004

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