Details
Category: Volume 06 Issue 05 May 2018
Hits: 1547

Title: Anti- inflammatory response of soft tissue injury using different doses of low level laser application. (Experimental study on Rabbits)

Author: Dr Hareth. H. Kaskos

 DOI:  https://dx.doi.org/10.18535/jmscr/v6i5.02

Abstract

Aim of study: To evaluate the effect of different wave length of low level laser therapy on the degree of inflammation following soft tissue injury.

Materials & Methods: Nine healthy rabbits aged (6-10) months ,weights 1-1.5 Kg were introduced to surgical operation by incision of 2cm of skin and subcutaneous tissue on the dorsum of rabbit ,the animals were divided into three groups .The first group(control) applied the same surgical procedure but without laser irradiation ,the second group irradiated by 650nm,the third group irradiated by 810nm,the exposure time was(1mint) at 2.5 mw immediately after surgery and then at day 2,3,5,7 post operative days .The experimental animals were scarified at the subsequent postoperative days and the specimens were examined histopatholgically. Number .of inflammatory cells were calculated  statistically to assess the degree of inflammatory response.

Results: The animals irradiated with 650nm of laser were significantly differences in all post operative days  compare to control but non significant differences in the group treated with 810nm of laser except in 7 postoperative days compare to control .Statistically comparison of both treated groups(650,810nm) showed significant differences in all post operative days.

Conclusion: laser reduce the inflammation and enhance the healing by increase the inflammatory cells at wave length of 650nm but induced damage (reduction of healing)at 810nm.

Keywords: different wave length ,laser ,soft tissue healing.

References

  1. Antonio, PI.Gyselle, ME. Alessandro, VI.Darcy, AL.Carolina, CA.Jean, S.: Phototherapy improves healing of cutaneous wound in nourished and unnourished Wistar Rats. Braz Dent J.2004.Vol 15 .Special issue.
  2. D.R. Padma,P. Chaya, M. David. G. Dinesh,D.: Photobiomodulation current concept and its application in dentistry .2013. International Journal of Dental Research, 1 (2) PP25-29
  3. Kathrin, H. Beckmann, GM. And Sven,S.: Low Level Laser Therapy for the Treatment of Diabetic Foot Ulcers: A Critical Survey.2014.Evidence-Based Complementary and Alternative Medicine Vol 2014.
  4. Richard,M. BS, CLT.: Laser-Accelerated Inflammation/Pain Reduction And Healing: Practical Pain Management, Nov/Dec 2003
  5. Ying-Ying, H.Aaron, CH. Chen, JD. Carroll, MR. Hamblin: biphasic dose response in low level light therapy. 2009. Inmternational dose response society.
  6. Sutherland JC.: Biological effects of polychromatic light. Photochem  Photobiol . 2002.76:164-70
  7. Prado, RP. Pinfildi, CE.Liebano, RE. Hochaman, BS. Ferreira, LM.: Diode laser in viability of random skin flap in rats . 2005.Photomed Laser Surg 23:115.
  1. Bjordal, JM. Johnson, MI. Lopes-Martins, RA. Bogen, B. Chow, R. and Ljunggren, AE.: Short-term efficacy of physical interventions in osteoarthritic knee pain. A systematic review and meta analysis of randomized placebo-controlled trials. 2007. BMC Musculoskelet Disord 8:51.
  2. toma,C. Marian, RC. : The effect of low level laser therapy on surgical wound healing. Romanian Reports in Physics, 2010.Vol. 62, No. 3, PP. 617–627.
  3. Kaviani, A.Djavid, GE.Ataie Fashtamil, L.Fateh, M.Ghodsi, M.Salami, M.Zand, N.Kashef, N.Larijani, B.:Randamized clinical trail the effect of low level laser therapy on chronic diabetic wound healing-preliminary report .Photo Med Laser surg.2011.
  4. Hawkins, D.Abrahamse, H.: Effect of multiple exposures of low-level laser therapy on the cellular responses of wounded human skin fibroblasts. Photomed Laser Surg. 2006 Dec;24(6) :PP.705-714.
  1. Bostanciklioglu, M. Demiryürek ,ŞCB. Demir,T,. Öztuzcu,S. Aras, MH. Özsevik, S. Usumez, A. Ergün, S. Özbal, HK. Bagci, C.: Assessment of the effect of laser irradiations at different wavelengths (660, 810, 980, and 1064 nm) on autophagy in a rat model of mucositis. Lasers Med Sci. 2015 May;30(4):PP1289-1295.
  2. Albertini, R.Villaverde, AB. Aimbire, F. Salgado, MA Bjordal, JM. Alves, LP. Munin, E. Costa, MS.: Anti-inflammatory effects of low-level laser therapy (LLLT) with two different red wavelengths (660 nm and 684 nm) in carrageenan-induced rat paw edema.J Photochem Photobiol B. 2007 Nov 12;89(1):PP50-55.
  3. Usumez, A. Cengiz, B.Oztuzcu, S. Demir, T. Aras, MH. Gutknecht, N: Effects of laser irradiation at different wavelengths (660, 810, 980, and 1,064 nm) on mucositis in an animal model of wound healing. Lasers Med Sci. 2014 Nov;29(6):PP1807-1813.
  4. Nicklas, W., Baneux, P.Boot, R. Decelle, T. Deeny, AA. Fumanelli, M., Illgen-Wilcke B; FELASA (Federation of European Animal Science Associations Working Group in Health Monitoring of Rodent and Rabbit Colonies) Recommendations for the health monitoring of rodent and rabbit colonies in breeding and experimental units. 2002.Laboratory animals 36, 20-42.
  1. H, lizarelli. RFZ, Ramalho, LTO: Effect of LLLT on bone repair histological study in rats.2007laserin surgery and medicine.39:788-796.
  2. Poon,V.K. Huang, L.and Burd,A.: Biostimulation of dermal fibroblast by sublethal Q-switched Nd:YAG532 nm laser: collagen remodeling and pigmentation, J Photochem Photobiol B 81 (2005) 1-8.
  3. Al-Watban FAH, Zhang XY.: The acceleration of wound healingis not attributed to laser skin transmission. Laser Therapy2000;12:3-11.
  4. Corazza, AV.Jorge, J. Kurachi, C. Bagnato, V.: Photobiomodulationon the angiogenesis of skin wounds in rats usingdifferent light sources. (2007) Photomed Laser Surg 25:102–106
  5. Kathrin, H. Beckmann, GMH. and Sven,S.: Low Level Laser Therapy for the Treatment of Diabetic Foot Ulcers: A Critical Survey. Evidence-Based Complementary and Alternative Medicine Vol 2014 , Art.
  1. Lubart, R. Wollman,Y. Friedmann,H. Rochkind, S.Laulicht, I.: Effects of visible and near-infraredlasers on cell cultures. J Photochem Photobiol 1992; 12:305–310.
  2. Brassolatti, P.Bossini, PS.Oliveira, MC.Kido, HW.Tim, CR.Almeidalopes, L.Retto Dasilva DEA vol, Araujo-Moreira, FM.Parizotto,NA.:Comparative effect of two different doses of low level laser therapy on wound healing third degree burns in rats. 2016.Microse Res Tech. Feb(8).

Corresponding Author

Assist.Prof. *Dr Hareth. H. Kaskos

MS.c Oral & Maxillofacial Surgeon–Duhok Polytechnic University – Iraq-Kurdistan Region