Title: Draft Genome Sequence of antagonistic Streptomyces sp. Ru87 Isolated from Egyptian Soil
Authors: Dina H. Amin, Chiara Borsetto, Assem Abolmaaty, Sahar Tolba, Nagwa A. Abdallah, Elizabeth M. H. Wellington
DOI: https://dx.doi.org/10.18535/jmscr/v5i11.69
Abstract
Streptomyces sp. Ru87 was selectively isolated from Egyptian clay soil by sucrose gradient centrifugation method. It recorded antagonistic activity against some food and blood borne pathogens such as Staphylococcus aureus ATCC 6538, Pseudomonas aeruginosa ATCC 10145, Streptococcus mutans ATCC 25175 and Escherichia coli ATCC 51659. NRPS and PKS PCR assays revealed that our strain harbors Non ribosomal peptide synthases and polyketide synthases biosynthetic gene clusters. We reported here the draft genome sequence of strain Streptomyces sp. Ru87. The draft genome was 7, 662, 503 bp, with an average GC content of 73.12%was assembled in 629contigs using Illumina MiSeq and HiSeq 2500 platforms using 2X250bp paired-end technology. NCBI prokaryote pipeline annotation identified 6,527 coding sequences (CDS) with 6,051 coding genes, 3 recorded non-coding RNAs and 74 RNA genes with 62tRNAs. Previous data clarified, the significance of Streptomyces sp. Ru87in antibiotic production and drug discovery against resistant pathogens.
Keywords: Streptomyces-Antimicrobial-Next generation sequencing-Illumina platform-food borne pathogens.
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