Title: Microbial Identification and Sequencing of Soil Bacteria from Indogangatic Plain

Authors: Priyanka, Chandrawati Jee

 DOI:  https://dx.doi.org/10.18535/jmscr/v5i7.167

Abstract

Motive of the present experimentation is to carryout Microbial Identification And Sequencing of Soil Bacteria from Indogangatic Plain. Systematic and scientific investigation was conducted for isolation, identification and characterization of selected soil bacteria. Identification was made on the basis of 16S rRNA sequencing. 16S rRNA sequencing showed that out of all 9 isolates;5 Bacillus sp., 1 achromobacter sp., 1 Brevundimonas sp., 1 Pseudomonas sp. and 1 Ochrobactrum sp. were identified. The further investigation shows that the isolates from Patna region of Indogangatic plain is having inhibitory effect on both Gram positive and Gram negative Bacteria. On the basis of sequencing, phylogenetic tree of each isolates was prepared and a comparative study was made.

Keywords: Microbial identification, Indogangatic plain, 16S rRNA sequencing, Phylogenetic tree, soil bacteria.

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