Title: Frequency of Rh Phenotypes in Voluntary Blood Donors
Authors: Dr Rashi Pachaury, Dev Raj Arya, Novrang Lal Mahawar, Arun Bharti, Pankaj Kumar Das
DOI: https://dx.doi.org/10.18535/jmscr/v5i7.129
Abstract
Background: Very few reports are available regarding Rh phenotypes prevalence in India and no reports are available from Rajasthan. There is a possibility of alloimmunization and antibody production in the recipients, even after proper grouping and cross matching. Due to the heavy financial burden of complete phenotyping; the knowledge of Rh phenotypes can play a major role in preventing alloimmunization. The aim of this study to prevalence of principal Rh blood group antigens like D, C, E, c & e in the voluntary blood donors with a view to generate blood bank data for constitution of panel of blood donors for multipurpose utilities.
Materials and Methods: A prospective study was carried out on 3014 healthy blood donors from April 2016 to Nov 2016 at our blood bank. Donors were grouped and typed for ABO and Rh major antigens. Statistical analysis was carried out using Microsoft excel software. Incidence was given in proportion with 95% confidence interval. Allele frequencies were also calculated using Hardy-Weinberg principal.
Results: Among Rh antigens, e was the most common antigen (98.71%), followed by D-91.94%, C-81.85%, c-62.77% and E-17.25% with DCe/DCe (R1R1) (36.43%) being the most common phenotype and the least common phenotype is r’’r’, r”r”, r’r” (0.03%).
Conclusion: Determination of Rh phenotypes can play a major role in preventing alloimmunization in multi-transfusion cases. Database for antigen frequency to at least Rh blood group system in local donor population helps to provide antigen negative blood unit to patients with multiple alloantibodies, minimize alloimmunization rate, there by improve blood safety.
Keywords: Rh blood group antigens, Rh phenotypes, Allele, Prevalence, Alloimmunization
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