Title: Hepatoprotective Potential of Russelia Equisetiformis Plant Extract on Drug-Induced Hepatotoxicity in Experimental Models
Authors: Ojokuku Hammed Olusegun, Adedokun Kamoru Ademola, Shittu Mujeeb Olushola, Awe Emmanuel O., Oyenike Musiliu A.
DOI: https://dx.doi.org/10.18535/jmscr/v5i7.85
Abstract
Background: A large number of herbal medicines are widely in-use to offer relief from hepatic injury, although attempts are still ongoing globally to get scientific proofs for these orthodox remedies, there is still paucity of data on Russelia equisetiformes. There are solitary reports of anti-inflammatory, analgesic, and antinociceptive properties. Meanwhile, inflammation is a hallmark of hepatic injury. Further study may pave way for next generation hepatoprotective medication.
Methods: The experimental animals were grouped into five (5), with six animals each. Groups 3, 4 and 5 were orally pretreated with the extracts for 7 days at doses of 100, 200 and 400 mg/kg, while groups 1 and 2 served as normal (negative) and paracetamol-induced (PCM-induced;positive) controls. Liver damage was induced on the 8th day using 2000 mg/kg of paracetamol. Liver enzymes such as alanine amino transaminase (ALT) aspartate amino transaminase (AST) and alkaline phosphatase (ALP), along with other liver function tests [plasma total protein (TP), albumin (ALB) and bilirubin (BIL)] were subsequently estimated. Liver samples were examined for histopathological changes.
Results: Comparison of case groups with positive controls shows that levels of biomarkers such as AST, ALT, ALP were significantly reduced (p<0.05) at a dose of 400mg/kg of the extract. Serum proteins were also significantly elevated (p<0.05). Histologically, there were significant changes in the hepatic cellular architecture of the PCM-induced group compared with extract-treated groups.
Conclusion: The results obtained from this study show that Russelia equisetiformes may offer hepatoprotective influence against any liver damage as a result of drug toxicity.
Keywords: Hepatoprotective Influence; Histopathological Changes; Liver Function Tests; Paracetamol; Plant-Extract; Russelia equisetiformes.
References
1. A. Keiron, History, Scope & Importance of Medicinal Plants. http://www.eHow.com. 2012. (Accessed 08.04.17)
2. S. Verma, N. Kaplowitz,Diagnosis, management and prevention of drug-induced liver injury. Gut. 58(2009), 1555-1564.
3. T.K. Chatterjee, Medicinal Plants with Hepatoprotective Properties in Herbal Options, (2000).
4. R.K. Dhiman, Y.K. Chawla, Herbal medicines for liver diseases. Dig Dis Sci., 50(2005), 1807. doi:10.1007/s10620-005-2942-9.
5. S.A. Tammana, National seminar on Unani Medicine, Aligarh: Faculty of Unani Medicine, Aligarh Muslim University. (1990).
6. A.L. Babson, S.J. Greeley, C.M. Coleman, G.D. Phillip, Phenolphthalein monoph-osphate as a substrate for serum alkaline phosphatase. Clinical Chemistry. 12 (1966), 481-490.
7. E.O. Awe, A.Adeloye, J.M. Makinde, Anti-inflammatory activities of Russelia equisetiformis, Schlecht and cham: Identification of its active cconstituents.J Intercult Ethnopharmacol. 1(2012), 25-29.
8. F.O. Oladeinde, In: African Healing Wisdom: From tradition to current application and research. International Conference Washington D.C. (2005).
9. O.T. Kolawole, O.S. Kolawole, Effects of Russelia equisetiformis methanol and aqueous extracts on hepatic function indices. Journal of Biology and Medicine, 2 (2010), (3): 38-41,
10. E.O. Awe, J.M. Makinde,O.A. Adeloye, S.O. Banjoko, Membrane stabilizing activity of Russelia equisetiformis, Schlecht & Chan., J. Nat. Prod., 2 (2009), 3-9.
11. O.T Kolawole, M.J. Makinde, O.A. Olajide, Central nervous system depressant activity of Russelia equisetiformis. Nigerian Journal of Physiological Sciences. (2007).
12. E.O. Awe, A. Adeloye, T. Idowu, O.A Olajide, J.M. Makinde, Antinociceptive effect of Russelia equisetiformis leave extracts: Identification of its active constituents. Phytomedicine. 15 (2008), 301-305.
13. D. Burns, W.F. Reynolds, P.B Reese, R.G Enriquez, Phytochemical screening of Russelia equisetiformis. Magn. Reson. Chem.38(2000), 488 -493
14. E.O. Awe, J.M Makinde, O.A Olajide, O.K Wakeel, Evaluation of the anti-inflammatory and analgesic properties of the extract of Russelia equisetiformis (Schlecht & Cham) Scrophulariacae. Inflammopharmacology. 12 (2004), 399-405.
15. C.E Johnson, L. Long-Ze, J.M.F. Harnly, O. Oladeinde,Identification of the Phenolic Components of Vernonia amygdalina and Russelia equisetiformis.Journal of Natural Products, (2011), 457-64.
16. H.U. Bergmeyer, M. Horder, R. Rej, Approved recommendation (1985) on IFCC methods for the measurement of catalytic concentration of enzymes. Journal of Clinical Chemistry and Clinical Biochemistry. 24(1986), 481-489.
17. C.C. Garber, Jendrassik-Grof analysis for total and direct bilirubin in serum with a centrifugal analyzer. Clinical Chemistry, 27(1981), 1410-6.
18. B.T. Doumas, W.A Watson, H.G. Biggs, Albumin standards and themeasurement of serum albumin with bromocresol green. Clin.ChemActa 31(1971), 87-96.
19. S.R. Kingsley, S.J. Frankel, The determination of serum total protein, albumin and globulin by Biuret reaction. Journal of Biology & Chemistry, 128(1939), 131-137.
20. C. Mayuren, V.V. Reddy, S.V. Priya, V. A. Devi, Protective effect of livactine against CCL4 and paracetamol induced hepatotoxicity in adult wister rats. North Am J Med Sci. 2 (2010), 491-495.
21. N.P.F. Vermeulen, J.G.M. Bessems, S.R. Vande, Molecular aspects of paracetamol hepatotoxicity and it mechanism based prevention. Drug Metab Rev 24(1992), 367-407.
22. E.M. Willianson,D.T. Okpako, F.J. Evans, Selection, preparation and pharmacological evaluation of plant material, John Wiley, England. (1996).
23. H. Chen, X. Yan, P. Zhu and J. Ling, Antioxidant activity and hepatoprotective potential of Agarose-ligosaccharide in vitro and in vivo, (2006).
24. C.G. Heijnen, G.R. Haenen, F.A.Van Acter, Flavonoids as peroxynitrite scavengers: the role of the hydroxyl group. Toxicol in vitro. Pubmed. 15(2001), (1):3-6.
25. E.O.Awe, J.M. Makinde, O.A. Adeloye, S.O. Banjoko,Free radical scavenging; a possible mechanism for anti-inflammatory activity of Russelia equisetiformis, Schlecht & Chan., Inflammophar-macology. 4 (2010),179-85.