Title: Amplification of Hsp 65 Gene and Usage of Restriction Endonuclease for Identification of Non tuberculous Rapid Grower Mycobacterium
Authors: Ajoy Kumar Verma, Rohit Sarin, Vijay Kumar Arora, Gavish Kumar, Jyoti Arora, Paras Singh, Vithal Prasad Myneedu
DOI: https://dx.doi.org/10.18535/jmscr/v5i4.133
Abstract
The rapid grower mycobacteria have emerged as significant group of human pathogen amongst the Runyon group IV organisms that are capable of causing infection in both the healthy and immunocompromised hosts. In this study, we analyzed and identified 121 rapidly growing mycobacterium isolated from clinical samples by polymerase chain reaction – restriction enzyme analysis (PRA) at a national reference laboratory. The results were analyzed and compared with standard biochemical test.
In this study, 8 different types of rapid grower mycobacteria were identified by analyzing the fragment generated through restriction enzymes. More than 50% of the isolates were from the pulmonary samples sputum. In pulmonary as well as extrapulmonary samples the most common isolate was M. chelonae (57/121). All strains of M. chelonae were having the same band fragments size. The others species identified in this study were M.fortuitum (42), M. abscessus (11), M. immunogen (06), M. peregrinum (02), M. smegmatis (01), M. wolinskyi (01), M.goodii(01). The study showed that in pulmonary as well as extrapulmonary sample M. chelonae was the most common isolate.
PCR- REA is a rapid accurate system with concordance of 119/121 (98.34%) when compared to standard biochemical tests for identification of clinically important species of rapidly growing mycobacterium.
Keywords: Rapid grower mycobacterium;REA; PCR.
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