Title: Evaluation of Antibacterial and Cytoxicity Activities of 5-Nitro Acetyl Salicylic Acid and 5-Bromo Acetyl Salicylic Acid Compounds
Authors: Sattar Jabbar Ahmed Al-Salman, Majda Ismaeel Abd- Al-Majeed, Ghaida J. Al-Ghizawi, Abbas Dawwas Matter Al-Maliki
DOI: https://dx.doi.org/10.18535/jmscr/v5i3.196
Abstract
Introduction: Phenolic compounds have dramatic effects in treat different various diseases caused by pathogenic bacteria. From these compounds, substituted phenolic carboxylic acids carrying active chemical groups. Therefore the currently research was carried out to investigate and evaluate the antibacterial activity of 5-nitro acetyl salicylic acid and 5-bromo acetyl salicylic acid.
Material and Methods: Previously substituted carboxylic acids were biochemically tested by using the concentration of 0.1,1.0,10, and 100mg/ml of both 5-nitro acetyl salicylic acid and 5-bromo acetyl salicylic acid against two pathogenic bacteria which were represented by Escherichia coli and Staphylococcus aurous. Also cytoxicity activities of the two phenolic carboxylic acids were estimated and evaluated by using hemolysis method of red blood cells.
Results: The concentration of 0.1,1.0,10,and 100mg/ml belonging to 5-nitro acetyl salicylic acid recorded inhibition zone diameters equal 8,23, 25 and 35mm against growth of Escherichia coli bacteria and Staphylococcus aurous respectively. Whereas the same concentration belonging to 5-bromo acetyl salicylic acid recorded 14,20,9 and 30mm and 19,22,15,and 20mm against growth of Escherichia coli and Staphylococcus aurous bacteria. Concerning cytoxicity activity of both 5-nitro acetyl salicylic acid and 5- bromo acetyl salicylic acid, there were no hemolytic results at concentration of 1:10,1:100,and 1:1000mg/ml.
Conclusion: The two phenolic acids represented by 5-nitro acetyl salicylic acid and 5-bromo acetyl salicylic acid showed excellent antibacterial activities against pathogenic bacteria. Also these acids had no toxic effect against red blood cells so these active phenolic acids can be used safety to treat various diseases caused by these micro-organisms.
Keywords: Antibacterial activity, Cytoxicity, 5-nitro acetyl salicylic acid, 5-bromo acetyl ,salicylic acid, phenolic group.
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