Title: Oral Mucosal Changes in Tobacco Users- A Cytomorphometric Analysis
Authors: N. Mohan, Anju Mathew, Sabitha Gokulraj
DOI: https://dx.doi.org/10.18535/jmscr/v5i2.71
Abstract
Aim: To assess the effect of tobacco smoking and tobacco chewing on the buccal mucosa by cytomorphometry.
Materials and Methodology: Oral mucosal cells were collected from 45 subjects divided into 3 groups: Tobacco Smokers, Tobacco Chewers and Controls. The cells were stained using Papanicolaou stain and viewed under high power microscope. Cytomorphometric analyses of the cells in captured image were analysed using ImageJ analysis software to find out the Cellular Diameter, Nuclear Diameter and Nuclear Cytoplasmic Ratio. The statistical test used was ANOVA and p<0.05 was considered significant.
Results: The cellular diameter gradually decreases while nuclear diameter and nuclear cytoplasmic ratio increases from controls to smokers and to chewers.
Conclusion: The results confirmed that tobacco using influenced the cytomorphology of normal buccal mucosa and the degree of these changes was found to be greater in chewers compared to smokers. Cytomorphometric changes can be an early indicator of cellular alterations.
Keywords: Cytomorphometry, Exfoliative Cytology, Cellular diameter, Nuclear Diameter, Nuclear Cytoplasmic Ratio
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