Title: Comparative Study of Demonstration of Mycobacterium Tuberculosis on Tissue by Concentration Bleach Method and Auramine Rhodamine with Ziehl Neelsen Staining– An Autopsy Study
Authors: Anjali Bode, Vikas Kavishwar
DOI: https://dx.doi.org/10.18535/jmscr/v5i2.40
Abstract
Objectives: Diagnosis of tuberculosis is depending upon to demonstrate the bacilli in tissue section or cultivate the bacilli. Objectives of our study were to compare demonstration of acid fast bacilli by modified concentration bleach method and Auramine Rhodamine fluorescent stain with conventional Ziehl-Neelsen staining on autopsy tissue material.
Method: Study was conducted in tertiary care hospital in Mumbai of India .It was three year prospective autopsy study .Total 887 clinical autopsies had been performed during three year. Out of them 108 cases were of tuberculosis. Tissue from tubercular lesions were subjected for routine Ziehl-Neelsen stain, concentration bleach and Fluorescent staining. Chi square test was applied for significance of study.
Results: Out of 108 cases proportion of acid fast bacilli positive cases were maximum by Auramine Rhodamine staining method 74%, followed by concentration bleach method 45 % and conventional Ziehl - Neelsen staining 25 %.
Conclusions: Fluorescent staining has more advantages than concentration bleach and Ziehl Neelsen staining method in detecting tubercular bacilli. Still institutes who don’t have fluorescent microscope can used concentration bleach method because it definitely increases rate of positivity on tissue.
Keywords– autopsy tissue, tuberculosis, Ziehl Neelsen stain, Concentration bleach method, Auramine – Rhodamine fluorescent stain.
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