Title: Detection of Various Beta Lactamases in Pseudomonas Aeruginosa from Various Clinical Samples and Their Co-Existance
Authors: Gagan Priya Pandey, Akansha Rana, Dr Kush Manna, Naval Kishor Karn
DOI: https://dx.doi.org/10.18535/jmscr/v5i1.54
Abstract
In the present study, it’s to be detect various type of beta lactamase which affect the growth of Pseudomonas aeruginosa. Pseudomonas aeruginosa is leading nosocomial infection agent. Treatment is more complicated because of high degree of resistance against beta lactamases enzymes. Samples i.e., urine, body fluids, pus, sputum, ear swabs, etc. were collected and Nutrient Agar, Blood Agar and MacConkey agar, oxidase test and their biochmecial reactions used for colony growth and identification. Among various samples, urine, sputum, pus and ET secreation has been detected from ESBL, AmpC and MBL producer Pseudomonas aeruginosa. Maximum ESBL production for 41-50 aged group patients and for AmpC and MBL, the aged group are <10 and 50-60 years respectively. Out of all the positive samples, only one sample i.e., AmpC-MBL, has been isolated for co-existance. It’s useful for the treatment against Pseudomonas aeruginosal infection for the physician and restrict the growth of such common and deadly infection.
Keywords: Beta-Lactamase, ESBL, AmpC, MBL, Pseudomonas aeruginosa.
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