Title: Isolation of Extended Spectrum Β-Lactamases in Urine Samples
Authors: Sukesh Kumar B Y, Ayesha Begum, Syed Haroon Hussain, Konuri Sridhar
DOI: http://dx.doi.org/10.18535/jmscr/v3i8.07
DIDS : 08.2015-88636192
Background: Extended spectrum β-lactamase (ESBL) production among uropathogens is an important marker of endemicity. Materials and Methods: During the study period of 7 months, 960 urine samples were processed for significant bacteriuria. Results: Out of these 960 Urine samples 611 (63.3%) showed significant growth. Among these E.coli and klebsiella species has highest prevalence of ESBL. Maximum number of ESBL producers were seen in the age group of 40 – 60 years. Females show higher percentage of ESBL producers compared to males. Conclusion: This study reveals the prevalence of ESBL producing organisms is a significant uropathogen in our area. Constant revision of antibiotic policies with infection control interventions is suggested. Keywords: ESBL, Endemicity, Infection, Uropathogens. 1. Philippon A, Labia R, Jacoby G. Extended-spectrum beta-lactamases. Antimicrob Agents Chemother. 1989;33:1131–6. 2. Nathisuwan S, Burgess DS, Lewis JS., 2nd Extended Spectrum β-lactamases: Epidemiology, Detection and treatment. Pharmacotherapy. 2001;21:920–8. 3. Ananthkrishnan AN, Kanungo R, Kumar A, Badrinath S. Detection of extended spectrum beta lactamase producers among surgical wound infections and burn patients in JIPMER. Ind J Med Microbiol. 2000;18:160–5. 4. Brigante G, Luzzaro F, Perilli M, Lombardi G, Colì A, Rossolini GM, et al. Evolution of CTX-M type beta -lactamasees in isolates of Escherichia coli infecting hospital and community patients. Int J Antimicrob Agents. 2005;25:157–62. 5. Subha A, Ananthan S. Extended spectrum beta lactamase (ESBL) mediated resistance to third generation cephalosporins among Klebsiella pneumoniae in Chennai. Indian J Med Microbiol. 2002;20:92–5. 6. Tankhiwale SS, Jalgaonkar SV, Ahamad S, Hassani U.Evalutaion of extended spectrum beta lactamase in Urinary Isolates. Indian J Med Res 2004;120:553-6 7. Hasan Ejaz1, Ikram-ul-Haq1, Aizza Zafar, Saqib Mahmood:Urinary tract infections caused by extended spectrum β-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniaeAfrican Journal of Biotechnology 2011:10(73),16661-16666, 8. T.Menon, D Bindu, CPG Kumar, S Nalini, MA Thirunarayan, Comparison of double disc and three dimensional methods to screen for ESBL producers in a tertiary care hospital. Indian Journal of Medical Microbiology, 2006;24(2):117-120. 9. Akram M, Shahid M, Khan AU, Etiology and antibiotic resistance patterns of community acquired urinary tract infections in JNMC Hospital, Aligarh, India. Ann Clin Microbiol Antimicrob 2007;6:4 10. Ritu Agarwal, Uma Chowdhary, Rama Sikka Indian Journal Medical,Microbiology (2004; 2:75-80) 11. Wong ES, Stamm WE. Sexual acquisition of urinary tract infection in man. J Am Med Assoc 1983;250:3087-8 12. C Rodrigues, P Joshi, SH Jani, M Alphonse, R Radhakrishnan, A Mehta: Indian Journal of Medical Microbiology, (2004) 22 (4): 247-250Abstract
References