Title: Possible Applications of Nucleotidases in B-Chronic Lymphocytic Leukemia Patients; Egyptian Single Centre Study
Authors: Youssef SR, Farweez BA, Salah Al-din YM, Ayoub MS
DOI: http://dx.doi.org/10.18535/jmscr/v3i10.32
Abstract
The high variability of chronic lymphocytic leukemia (CLL) clinical presentation and disease course has been linked to availability of extracellular nucleotides and nucleosides under control of enzymes as CD39 and CD73. In this work we aimed to study the relation of CD39 and CD73 expression by B-CLL cells to the patients' clinical condition and their relationship with disease prognostic parameters and patients' outcome, aiming to apply them in routine clinical practice. The study included 40 newly diagnosed CLL patients and 20 controls. Quantification of CD73 and CD39 expression by CD19 positive cells was determined by flowcytometric analysis. A statistically significant lower percentage of lymphocytes expressing CD73 and higher ratio of CD39/CD73 in patients compared to controls were found. The percentage of cells expressing CD39 was higher in patients with lower tumor load, having a stable disease course and lymphocyte doubling time (LDT) >6 months. A cutoff point of <19% for CD73 expression, >2 for CD39/CD73ratio confirmed the diagnosis of CLL and a cutoff point > 41% for CD39 expression predicted clinically beneficial response to treatment. Thus, it is applicable to incorporate the detection of CD73 expression and the CD39/CD73 ratio into the CLL diagnostic panel and the use of CD39 as a prognostic marker for CLL.
Key Words: Nucleotidases; B-CLL; CD73; CD39; ATP; Prognostic Factor; DiagnosticFactor
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