Title: Study on the Phytochemical Analysis and Antioxidants, Antimicrobial Activity of Medicinal Plants against Fish Pathogen
Authors: Thamarai Sridevi,.K, J.Poovizhi, Dr V.Senthil Kumar
DOI: http://dx.doi.org/10.18535/jmscr/v3i10.15
Abstract
The present study was designed to evaluate the phytochemical screening, antioxidants and antimicrobial activities of different plants extracts. Phytochemical screening revealed the presence of terphenols, phenols, quinone, carbohydrates, flavonoid, tannin, saponion, and also screening total content of flavonoid and tannin ,phenol, and antioxidant capacity are extract the plant part of leaves . The powdered plants are extracting with methanol further fractioned with hexane. The total content of tannin is higher in carciya papaya 1.963, total ascorbic acid content higher in carciya papaya. Free radical scavenging activities are higher in carciya papaya 1.642. The total phenol concentration is higher pomengrate. The total flavonoid content has higher in pomengrate and carciya papaya. The reducing power assays are higher in solanum nigrum 0.765. The microorganisms are isolated from fish pathogen. The antimicrobial activity is higher in pomengrate 19 ±0.1. Thin layer chromatography are used to separate the phytochemicals flavonoid, Caumarrins, alkaloid. After 24 hours plates were incubated and using MTT assay (2, 4 Terphenols tetrazolium). The inhibition areas are changed in pink or violet.
Keywords: Phytochemicals, antioxidant ,MIC,
Abbrevation: TLC-Thin layer chromatography,DPPH-1,1-diphenyl-2-picryl-hydrazyl
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