Title: Study on Platelet Count on the Basis of Red Cell: Platelet Ratio
Authors: Dr Ajamal Singh Bhayal, Dr Mahendra Kumar Gupta, Dr Narendra Kumar Sharma, Dr Asim Singh, Mr Manoj Kumar Yadav
DOI: http://dx.doi.org/10.18535/jmscr/v3i10.08
Abstract
The modern hematology analyzers are able to produce platelet counts with great precision and accuracy. Our aim to verification of the reliability of the estimation technique of platelet count on the basis of red cell: platelet ratio. An overnight fast venous blood samples was collected for the following parameters: Manual Method & Automated Method. The results of platelet count using the manual method were as follows: the range was between 100-499 x103/µl, the mean platelet count was 301.46±44.49 x 103/µl and by using the automated method, platelet count ranged between 95-484 x103/µl, the mean was 328.47±54.00 x103/µl. Red blood cell: platelet ratio method requires only an accurate RBC count performed on a calibrated hematology analyzer to calculate platelet count. This method is precise, simple and consumes less time than using a counting chamber and therefore, potentially should supersede ordinary manual counting.
KEY WORD: Red cell: platelet ratio, Manual Method & Automated Method
References
1. Bain BJ. Diagnosis from the blood smear. N Engl J Med 2005;353:498-570.
2. Gowland E, Kay Hem, Splillman JC, et al. Blood cell morphology in health and disease. In: Lewis SM, Bain BJ, Bates I, editors. Dacie and Philadelphia: Churchill Livingstone, 2006:79-113.
3. Meer W V, Mackenzie M A, Dinnissen J W and de Keijzer M H. Pseudoplatelets: a retrospective study of their incidence and interference with platelet counting J. Clin. Pathol. 2003;56:772-774.
4. International Council for Standardization in Haematology expert Panel on Cytometry. Platelet Counting by the RBC/Platelet Ratio Method. A “Reference Method. American journal of Clinical Pathology 2001;115;460-464.
5. Bain BJ, Lewis SM, Bates I. Basic haematological techniques. In: Lewis SM, Bain BJ, Bates I, editors. Dacie and Lewis: Practical Haematology. 10th ed. Philadelphia: Churchill Livingstone, 2006:26-54.
6. Charie LA, Harrison P, Smith CU, Cobb JR, Briggs C, Machin S. Accuracy in the low platelet count range: a comparison of automated platelet counts on Beckman Coulter high-volume hematology analyzers with the ISLH/ICSH platelet reference method. Lab Hematol 2001;7:236-44.
7. Rowan RM. Platelet counting and the assessment of platelet function. In:Koepke JA, editor. Practical Laboratory Hematology. New York, NY: Churchill Licvingston, 1999:57-70.
8. Latif S, CVeillon DM, Brown D, et al. Spurious automated platelet count: enumeration of yeast forms as platelets by the Cell-DYN 4000. Am J ClinPathol 2003;120:882-885.
9. Arnold JA, Jowri Z, Bain BJ. Candida glabrata in a blood smear. Br J Haematol 2001;104: 13-16.
10. Kakkar N. spurious rise in the automated platelet count because of bacteria. J ClinPathol 2004;57: 1096-1097.
11. K. H. Hong, M. J. Kim, K. W. Lee. Platelet count evaluation using three automated haematologyanalysers compared with the immunoplatelet reference method and estimation of possible inadequate platelet transfusion. Int. J. Lab. Hem. 2009,31, 298-306.
12. Harrison P., Horton A., Grant D., Briggs C. &MacHin S. Immunoplatelet counting: a proposed new reference procedure. British Journal Haematology 2000, 108.228-235.
13. Brown BA. Hematology: Principles and Procedures. 6th ed. Philadelphia: Lea and Febiger, 2000.
14. Mohapatra S, Pradhan BB, Satpathy UK, Mohanty A, Pattnaik JR. platelets-timatioin: its prognostic value in pregnancy induced hypertension. Indian J PhysiolPharmacol 2007;51:160-164.
15. Shrout PE, Fleiss JL. Intraclass correlations: uses in assessing rate reliability. Psychol Bull 1979’86:420-428. (Cited)
16. Portney LG, Watkins MP. Foundations of clinical research. Applications to practice. Norwalk, CT, USA: Appleton and Lange, 2002:509-514.
17. Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet 1986;1;307-310. (Cited)