Title: Assessment of Antioxidant activity in Brassica oleracea var. capitata rubra
Authors: Ayshwarya.M, Sudha Rameshwari.K
DOI: http://dx.doi.org/10.18535/jmscr/v3i12.17
Brassica oleracea var. capitata rubra extract with different solvents [methanol, ethanol. Water, acetone and chloroform] were analyzed for antioxidant activities. The antioxidant activities were evaluated based on the ability of the plant extracts to DPPH radical scavenging assay, FRAP assay, reductive activity, superoxide radical scavenging activity, Lipid peroxidation assay and Nitric oxide scavenging assay. Ascorbic acid is used as standard. It was found that methanol extract (70%) has potent antioxidant when compared to the other extracts. Ethanol and chloroform extract showed the second highest antioxidant activity (59%) followed by acetone extract (25%). Least activity was shown by water extract (22%) in DPPH assay. The results proved that the overall antioxidant activity were highest in methanol extract as compared to others The information of this study helpful to choose the solvent extracts for the further pharmacologic studies. Key words: Brassica oleracea var. capitata rubra, antioxidant activity, radical scavenging. 1. Halliwell B, and J.M.C. Gutteridge. (1989). Free radicals in biology and medicine. Clarendon Press, Oxford, pp. 23-30. 2. Gulcin, M.E. Buyukokuroglu, M. Oktay and O. I.Kufrevioglu. (2002)a. On the in vitro antioxidant properties of melatonin. J. Pineal Res. 33: 167-171. 3. Gulcin, M.Oktay, O.Kufrevio.lu and A.Aslan. (2002)b. Determination of antioxidant activity of lichen Cetraria islandica (L) Ach. J. Ethnopharmacol. 79: 325-329 4. Slater TF (1991). Antioxidant vitamins and –carotene in disease prevention. Am J Clin Nutr 53:189S 396S. 5. Mohammad I, Zafar I, Javid H, Hidayat H, Manzoor A, Asma E, Muhammad I.C (2009). Chemical constituents and antioxidant activity of Geranium Wallichianum. Rec.Nat. Prod. 3(4): 193-197 6. Lahvale, Manish S and Mishra S.H (2007). Evaluation of free radical scavenging activity of Butea monosperma Lam. Indian Journal of Experimental Biology.45: 376 7. Luximon-Ramma A, Bahorun T, Crozier A, Zbarsky V, Datla K.P, Dexter D.T, Aruoma O.I (2005). Characterization of the antioxidant functions of flavonoids and proanthocyanidins in Mauratian black teas. Food Res. Int. 38, 357–367. 8. Alizadeh H.A, Abbasi F, Liaghat A (2010). Evaluation of distribution uniformity and nitrate losses under furrow fertigation. J. Water Soil, 51, 20–31. 9. Mondal Susanta Kumar, Chakraborty Goutam, M Gupta & UK Mazumder (2006), In vitro antioxidant activity of Diospyros malabarica Kostel bark. Indian Journal of Experimental Biology, 44(1): 39-44. 10. Rajeshwar Yerra , GP Senthil Kumar, Gupta Malaya, Mazumder Upal Kanti (2005), studies on in-vitro antioxidant activities of methanol extracts of Mucuna pruriens (fabacae) seeds. European Bulletin of Drug Research., 13(1): 31-35 11. Health, R.L. and Packer, L. (1968) . Photoperoxidation in isolated chloroplasts 1. Kinetics and stoichiometry of fatty acid Peroxidatoin. Arch. Biochem. Biophys., 125, 189–198. 12. Adesegun.SA, A. Fajana, C. I. Orabueze, and H. A. B (2009). Coker, “Evaluation of antioxidant properties of Phaulopsis fascisepala C.B.Cl. (Acanthaceae),” Evidence-Based Complementary and Alternative Medicine, vol. 6, no. 2, pp. 227–231. 13. Husain SR, J. Cillard, and P. Cillard (1987), “Hydroxyl radical scavenging activity of flavonoids,” Phytochemistry, vol. 26, no. 9, pp. 2489–2491. 14. Afanaslev.I.B, A. I. Dorozhko, and A. V. Bordskii,(1989) “Chelating and free radical scavenging mechanisms of inhibitory action of rutin and quercetin in lipid peroxidation,” Biochemical Pharmacology, vol. 38, pp. 1763–1769. 15. Torel.J, J. Cillard, and P. Cillard, (1986)“Antioxidant activity of flavonoids and reactivity with peroxy radical,” Phytochemistry, vol. 25, no. 2, pp. 383–385. 16. Amit K. Jaiswal, Nissreen Abu-Ghannam and Shilpi Gupta (2011). A comparative study on the polyphenolic content, antibacterial activity and antioxidant capacity of different solvent extracts ofBrassica oleracea vegetables, International Journal of Food Science and Technology 17. Ekrem koksal (2008), ilhami gulcin. Antioxidant Activity of Cauliflower (Brassica oleracea L.). Turk J Agric For 32 65-78 c TUB.TAK 18. Shweta Saboo, Ritesh Tapadiya, S. S. Khadabadi and U. A. Deokate (2010). In vitro antioxidant activity and total phenolic, flavonoid contents of the crude extracts of Pterospermum acerifolium wild leaves (Sterculiaceae). J. Chem. Pharm. Res., 2(3):417-423. 19. Lucie Buricova, Mirjana Andjelkovic , Anna Cermakova1, Zuzana Rebl ova1, Ondrej Jurcek ,Erkki Kolehma inen , Roland Verhe and Frantisek Kvasn icka6(2011). Antioxidant Capacity and Antioxidants of Strawberry, Blackberry, and Raspberry Leaves. Czech J. Food Sci. Vol. 29, No. 2: 181–189. 20. Abhishek Mathur, Satish K. Verma, Reena Purohit, Vinay Gupta, VK Dua, GBKS Prasad, Deepika Mathur, Santosh K. Singh and Shivsaran Singh (2011). Evaluation of in vitro antimicrobial and antioxidant activities of peel and pulp of some citrus fruits. IJPI’S Journal of Biotechnology and Biotherape-utics. ISSN 2229 – 6824. Vol 1: 2.Abstract
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